FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1984-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1984-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14772
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG190.12862171676144057No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA180.12185215272136475No Hit
CTTTTGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTTGCTTATTAAGT150.10154346060113728No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA150.10154346060113728No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTT259.203149E-487.06
CCTTCTA259.203149E-487.09
TAACTTT400.00593383154.3756
CCAGCAT450.00945335348.3333329
AACATCA450.00945335348.333332145
AAAGTTT200.006038535429.070-74
GTTTATA400.00784857818.12575-79
Produced by FastQC (version 0.11.5)