FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1984-d10-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1984-d10-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033008
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC24980.24181806917274598No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG23950.23184718801790496No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG23810.23049192261821788No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT20850.20183773988197576No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG14910.14433576506667906No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA14570.14104440623886746No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA13390.1296214550129331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG6800.057.5760275
GTAGAAA12900.048.8974722
ACTGATC8550.048.333098
AGCGAAA2850.045.7958951
GTACTGA9000.044.3074766
GCGAAAG3550.040.846842
CAGGTAC9750.039.411973
ACAGTAT12450.038.4335448
GACAGTA11450.036.7247057
TAGAAAC17600.036.663523
AGGTACT10900.036.5841564
GCAGGTA11700.034.7023622
AGTAGAA18200.034.2630461
CAGTATG11850.034.2614339
CTGATCC12500.033.0598379
CGAAAGC4200.032.799043
GTCCGTA900.00362579532.226741
TTGGACA13050.032.223624
GGTCGTT2257.930794E-1032.222067
ACAAGGC14750.031.457478
Produced by FastQC (version 0.11.5)