FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1984-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1984-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences632475
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC15660.24759871931696906No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT10920.1726550456539784No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC10100.15969010632831337No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9190.1453021858571485No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT8090.12791019407881735No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA7830.12379935965848453No Hit
AAGCTGTGATGTGCAAGTGTTTTCTCTCGAGTGAATAAACATTGAGTGAC7650.1209533973674849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2200.046.1360131
CGAAAGC2200.039.5514073
GCTACTA1701.8280844E-938.382069
GCGAAAG2350.037.0268482
AGTCGAA800.002034546636.2554552
GTGATCG1251.2644203E-534.7997369
GTCGAAG950.00472251730.530913
AAGTCGA950.004726171530.5260851
TATACTG2802.6193447E-1028.486435
GTATTAG1606.824589E-527.1872921
GCAGTGC4450.026.0672171
ATAGCAC9600.024.1703033
CTACTGA4600.023.6448616
GGTCCTA6150.023.5770559
TAGCATG7700.022.6008032
CAACCGG2903.2823482E-722.499828145
GTTGACA4205.2750693E-1122.4403061
GTAATAT2603.2012886E-622.3075221
TCGAAGG1650.00259754221.9730034
AGCACCG7350.021.7039475
Produced by FastQC (version 0.11.5)