FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1983-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1983-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907004
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG2100.055.237619
GTTACGT1255.187758E-946.407271
TATACTG4950.035.153155
TTACGTG1551.4636462E-632.745262
AGCGAAA3000.031.4215871
CCTATAC1907.151897E-626.7117633
GCGAAAG3306.184564E-1126.3663122
TAGAGTG5500.026.3648595
GAGTGAT5600.025.8926327
TTAGAGT5850.024.7874744
ATACTGT7700.024.4816556
TACGTGT2552.705532E-622.7461533
CGTGTTG2903.2838943E-722.5010435
AGGTCGG4058.058123E-1021.4812938
GTAGCAT9800.021.4574411
GCGTGCG3055.3394797E-721.3932559
GTTGACA7500.021.2699991
CCCTATA2053.663564E-421.2216662
CGAAAGC4201.2660166E-920.7152463
TACTGTA3901.2192686E-820.448547
Produced by FastQC (version 0.11.5)