FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1983-d10-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1983-d10-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812376
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG14210.17491900302323063No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA10850.13355884467291007No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG9700.11940283809467538No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC9210.11337114833525364No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT9110.11214019124149409No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC8520.1048775443883128No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT8280.1019232473632899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG1255.1913958E-946.4027372
GTTACGT1453.8380676E-1045.0026551
TACTGCG1251.26428495E-534.8020555
TGCGTTA1251.2648181E-534.7999088
TACGTGT2103.783498E-1034.525853
GCGTTAA1301.654429E-533.4614529
AGCGAAA1751.008284E-733.1448141
CGTGTTG2257.912604E-1032.2241255
GGTGCGA900.003627879832.2221378
GTAACGG1703.0094143E-629.8547021
ACCGGCA1000.006072045329.001713
TGTACCG1255.15122E-429.001715
CATTCTA8350.028.6543872
ACGAACC3301.8189894E-1228.5622924
TTACGTG2354.292997E-827.7675972
CGAAAGC2104.981939E-727.6206783
GTAGCAT14200.027.572051
TTGATTA5650.026.946835145
GTTATGT7000.026.930161
CTAGACC1358.078615E-426.8534363
Produced by FastQC (version 0.11.5)