FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1983-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1983-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068616
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC20180.18884239053130406No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT12070.11294983417803964No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG2008.951247E-932.6249289
AGCGAAA3750.030.9361571
GCGGACC1958.755374E-626.0255839
TTACCGC1409.989715E-425.8952241
GCGAAAG4450.024.4392912
GAGCGAA1500.001493545724.1688731
ACTATAC4201.8189894E-1224.166618
GTATTAT7700.023.541111
CGAAAGC4700.023.1393283
TATTATA5500.022.4100882
CAACCCG3253.990681E-822.307642145
TATATAC5600.020.7152083
GATCGGA1750.0036589420.714248
GTATATA3901.2183591E-820.4505861
TAGACCC2556.9556714E-519.9028475
GGCGGAC2556.958174E-519.9019178
GTGTTCC16100.019.8136229
ATATACA6350.019.4103154
GTTGACA8000.019.0329881
CCACGAA3051.2745881E-519.017243
Produced by FastQC (version 0.11.5)