FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1983-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1983-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070534
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA131651.2297601010336898No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG126011.177076113416295No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA125141.1689493280923353No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG115961.0831977312257248No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT115481.0787139875987124No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG108521.0136997050070338No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT81830.7643848770800367No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT81090.7574724389883927No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC75610.7062830325800021No Hit
TCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAATGTATTGATA74560.6964748433959127No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT71000.6632204114955713No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC65570.6124980617149947No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA65260.6096023106225491No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT63210.590452988882184No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC60580.5658858102591791No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA57610.5381426465670404No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG56970.5321643217310239No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT56040.5234770684536876No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG48070.44902824198017066No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG48040.44874800800348236No Hit
GTATAAGGGAATGTGGTACTTATTGCATTTTGCGCTGGCACTTTTAAGAA45310.4232467161248498No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAA44700.4175486252655217No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG42270.39484967315377184No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA41370.38644265385312376No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG41260.38541512927193344No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGT40890.38195891022611145No Hit
GACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACACATCAG40700.3801840950404191No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC40190.3754201174367185No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT39410.3681340340428235No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT38830.3627161771601836No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA35830.3346927794913566No Hit
TCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACT32480.3033999854278332No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT32330.30199881554439184No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA32230.3010647022887643No Hit
ATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTT31840.2974216605918168No Hit
GGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAAGA31250.29191039238361416No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG31060.2901355771979218No Hit
GTGGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAA30930.28892122996560593No Hit
TCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCA30030.28051421066495785No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG28180.26323311543584793No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC28110.2625792361569086No Hit
ATCTTACGAAAGGGGAGAAAGCTAATGTATTGATAGGGCAAGGAGACGTA27990.26145830025015554No Hit
ATGCAATAAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCAT27610.25790866987877076No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC27320.25519974143745083No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG26990.2521171676938799No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA26970.25193034504275436No Hit
ATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACAC26510.24763342406686756No Hit
CACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAA25940.24230897850979044No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG25890.24184192188197667No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC25680.2398802840451588No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT25580.2389461707895312No Hit
CTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGA25080.2342756045113934No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA24900.23259420065126377No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA24720.23091279679113416No Hit
GCTGTTCTGTTGACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCT24310.2270829324430611No Hit
GAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATACTGACAAA23690.22129143025817022No Hit
CGCGAGATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAA23520.21970343772360337No Hit
TGTATTGATAGGGCAAGGAGACGTAGTGTTGGTAATGAAACGGAAACGGG23190.2166208639800324No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC22790.2128844109575221No Hit
TATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGA21640.20214210851780515No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA21470.20055411598323825No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC20340.18999863619464677No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA20200.1886908776367682No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG20020.1870094737766386No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG19960.18644900582326204No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA19680.18383348870750485No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT19550.182619141475189No Hit
GAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAAT19460.1817784395451242No Hit
GAGCAATCTTACGAAAGGGGAGAAAGCTAATGTATTGATAGGGCAAGGAG19020.1776683412203629No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG18980.1772946959181119No Hit
ATATGGAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA18910.1766408166391726No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG18770.175333058081294No Hit
TTACCAACACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCT18760.17523964675573125No Hit
ATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAATA18710.17477259012791746No Hit
ACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTTAA18630.17402529952341542No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAA18620.17393188819785266No Hit
ATCTCGCACTCGCGAGATACTGACAAAAACCACTGTGGATCATATGGCCA18570.1734648315700389No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTG18390.17178342770990926No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT18120.16926132191971485No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT18050.16860744264077554No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA18030.16842061998965002No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA17600.16440393299045147No Hit
GGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCG17520.16365664238594946No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA17310.16169500454913155No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA17190.16057406864237847No Hit
GAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAATGTATTGATAGGGCA17050.15926631008449987No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA17010.15889266478224887No Hit
CTTCTAAGTGTGTGCATATTGCAGCGAACTTATTTGTTTCAATTTTCGGG16860.1574914948988075No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC16700.15599691368980342No Hit
TCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATC16680.1558100910386779No Hit
CTGTGGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAG16570.15478256645748756No Hit
ATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCA16450.15366163055073448No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA16360.15282092862066968No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG16240.1516999927139166No Hit
GATATGGACCAGCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGG16110.15048564548160076No Hit
CAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA16060.150018588853787No Hit
GTATTGATAGGGCAAGGAGACGTAGTGTTGGTAATGAAACGGAAACGGGA15840.14796353969140635No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA15750.14712283776134152No Hit
ACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATT15620.14590849052902571No Hit
CATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTTAAT15570.14544143390121192No Hit
CTGCTGTTCTGTTGACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGG15520.14497437727339813No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCAT15460.1444139093200216No Hit
GTATTGAGAGGATTTCTAATCCTCGGCAAAGAAGACAAAAGATATGGACC15260.14254568280876648No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT15100.14105110159976236No Hit
TAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATACTG15050.1405840449719486No Hit
TTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAA15020.14030381099526032No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT15020.14030381099526032No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT14900.13918287508850724No Hit
GGGCTATATGGAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTT14890.13908946376294448No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT14880.13899605243738172No Hit
ATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGAAT14740.13768829387950313No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG14620.13656735797275005No Hit
ACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAG14610.1364739466471873No Hit
AACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAG14560.13600689001937352No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA14520.1356332447171225No Hit
GAGTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATG14430.13479254278705766No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACC14170.132363848322426No Hit
GGAGTAGAGTCTGCAGTATTGAGAGGATTTCTAATCCTCGGCAAAGAAGA14160.13227043699686325No Hit
GAATCCCGCCAGCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGG14050.13124291241567293No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA14020.13096267843898465No Hit
CTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTC14000.13077585578785914No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC13940.1302153878344826No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAT13930.13012197650891985No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG13930.13012197650891985No Hit
TTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATA13910.12993515385779433No Hit
TAAAAGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACTGGAGA13820.12909445192772953No Hit
GTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCC13820.12909445192772953No Hit
ACACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTA13640.1274130480675999No Hit
CTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTT13530.12638552348640958No Hit
GTTCATTGATGCTTAATGCTGGTCCATATCTTTTGTCTTCTTTGCCGAGG13520.12629211216084682No Hit
TCGCAATCTCGCACTCGCGAGATACTGACAAAAACCACTGTGGATCATAT13470.12582505553303303No Hit
CCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACAG13160.12292930444058758No Hit
GATTAAGAAAGAAGAATTTGCTGAGATCTTGAAGATCTGTTCCACCATTG13040.1218083685338345No Hit
TTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGTC12880.12031378732483042No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCG12830.11984673069701662No Hit
AAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTT12740.11900602876695182No Hit
CAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTA12720.11881920611582632No Hit
CTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGT12710.11872579479026354No Hit
GGATAGCAGTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGG12670.11835214948801252No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTATATGGGGGATCTCCAGTATAAG12640.11807191551132426No Hit
AAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCATCAT12550.11723121358125944No Hit
CCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACA12490.1166707456278829No Hit
CAATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCT12390.11573663237225534No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA12370.11554980972112983No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG12320.11508275309331603No Hit
CTCCAGTATAAGGGAATGTGGTACTTATTGCATTTTGCGCTGGCACTTTT12190.11386840586100021No Hit
CTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAG12160.11358817188431194No Hit
TACCAACACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTT11940.1115331227219313No Hit
ATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAACTAC11930.11143971139636853No Hit
GAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAA11800.11022536416405271No Hit
AGCTAATGTATTGATAGGGCAAGGAGACGTAGTGTTGGTAATGAAACGGA11690.10919783958286237No Hit
GTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAAT11690.10919783958286237No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATA11690.10919783958286237No Hit
GTCTAGGGCCCGAATTGATGCACGGATTGACTTCGAGTCTGGAAGGATTA11640.10873078295504861No Hit
GCTAATGTATTGATAGGGCAAGGAGACGTAGTGTTGGTAATGAAACGGAA11500.10742302439717001No Hit
AAGCTAATGTATTGATAGGGCAAGGAGACGTAGTGTTGGTAATGAAACGG11420.10667573379266797No Hit
TTTCTAATCCTCGGCAAAGAAGACAAAAGATATGGACCAGCATTAAGCAT11150.10415362800247355No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT11150.10415362800247355No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA11120.10387339402578527No Hit
ATATGGGGGATCTCCAGTATAAGGGAATGTGGTACTTATTGCATTTTGCG10930.10209857884009288No Hit
GATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGA10860.1014446995611536No Hit
GACGTAGTGTTGGTAATGAAACGGAAACGGGACTCTAGCATACTTACTGA10850.10135128823559085No Hit
GATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAAT10730.10023035232883777No Hit
GACCAGCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGGGAGAAA10710.10004352967771224No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA11650.0120.729542
AGCAGGT12000.0117.21921
CAGGTAC12100.0116.239593
AGTAGAA22700.0115.3080441
AGCGAAA1900.0110.668151
GGTACTG13650.0103.040235
GTACTGA14600.096.3355566
ACTGATC15150.093.3167658
TACTGAT15350.091.628627
CTGATCC15500.090.741889
GAATAAA25200.086.3175351
ATAAAAG25900.085.0964663
ACAAGGC16100.084.2080158
CAAGGCA16200.083.688229
AGAACTA26900.083.280628
GCGAAAG2600.080.8653262
TAAAAGA28250.078.787564
AAAGAAC28200.078.670166
AGCAGGC6600.075.79543
GCAGGCA6250.075.399954