FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1982_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1982_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734689
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG7950.10820905172120449No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA7680.10453402732312585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTG1800.052.3679432
GTTACGT2250.045.1169971
TACGTGT2100.044.8868063
TAACGTG700.001055779641.433985
GCGAAAG2400.039.2759551
CGAAAGC2700.034.911962
CGTGTTG2750.034.2772035
TCACGTA850.00273976134.1220972
CCGACTA2051.1363227E-831.8334224
ACGTGTT3100.030.4071944
AGCGAAA1701.0300141E-425.5915741
TGTTGGC10800.023.4952377
GTCGGGG4053.274181E-1123.2746432
GTATAAC1902.1904628E-422.8977241
GTGTTGG11900.022.5449586
CACCGAC2903.2794742E-722.5029372
TAGAGGT2953.8666258E-722.121534
GTATTAC2953.8666258E-722.121531
GTTGGCC11450.021.5282638
TACACTA2053.6619336E-421.222285
Produced by FastQC (version 0.11.5)