FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1982_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1982_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847385
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG12220.14420835865633685No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11600.13689173162139995No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA10070.11883618426099117No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA9320.10998542575098687No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG350.003564717562.1498875
GTTACGT1750.053.8632351
TTACGTG1800.052.3670352
CGTGTTG2300.040.9828955
GTTATGT5750.039.0913771
AGCGAAA3200.038.519981
TACGTGT2650.035.570063
TAGTCGG850.00274181634.117488
GCGAAAG4050.032.225872
GTGATCG900.003628007832.2220659
ACGTGTT3100.030.4066644
ACACCTA9900.029.2962441
CAACCCG2252.8161594E-828.999859145
CTACTGA11600.028.7532526
CATTCTA8850.028.675562
TACTGAA11550.028.2466147
TATACTG4200.027.622175
CGAAAGC5000.027.5531163
CTACATC4500.027.3919893
GATATAC1358.076351E-426.8548893
Produced by FastQC (version 0.11.5)