FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1982_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1982_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792041
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT1401.4139005E-841.428534
CAATACT2151.2732926E-1137.092994
GTGATCG1301.6543585E-533.461519
GCGAAAG3250.031.2327161
TCGGTGG1958.751542E-626.0256183
GTTATAG1701.0305913E-425.5898281
CCTACAC2601.14412614E-725.0961323
CGAAAGC4151.8189894E-1224.4593542
GTATATC1801.5192544E-424.168171
TAGAGTG2701.6479862E-724.1666455
TAGTACC4002.7284841E-1123.5624799
ATTATAC2502.2782406E-623.199983
TATACTG2903.2842945E-722.4999795
AGCGAAA2603.2010394E-622.309081
GAGCGAA1650.00259944721.9710661
TTAGAGT3055.337315E-721.3934234
CCGACTA1700.003092249221.323514
TATATAC4652.055458E-1020.26883
TCTAGAC3601.1745033E-720.1401422
TATAACC4402.2610038E-919.7727093
Produced by FastQC (version 0.11.5)