FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1982_d02_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1982_d02_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850024
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG14260.16775996913028338No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA10990.12929046709269384No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG9910.1165849434839487No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9720.11434971247870648No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC9630.11329091884464439No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT8780.10329120118961346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1051.1077645E-955.24131
GTTATGT4350.046.6693731
CAATCCG803.7341968E-545.312469
TTACGTG1401.4133548E-841.4309732
TAGTCGG907.4815274E-540.277758
GTTGACA6900.035.726711
GCTACTA2206.220944E-1032.954529
CCGTAGA1153.1606274E-431.5235671
TAGCATG11200.031.0732292
ACGTGTT1902.0759762E-730.5262934
ACATGGT7950.030.0943155
TTATGTG6750.029.0016822
GTATAAT1504.3962E-529.0016821
CCGACTA2303.486457E-828.3695414
CGTGTTG2156.122809E-726.976725
TATACTG2705.5606506E-926.8518315
GCTATAT1658.413791E-526.3651661
CTACTGA10450.026.3636156
GCATGTT13300.026.1653924
AGCATGT13400.025.9701293
Produced by FastQC (version 0.11.5)