FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1982-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1982-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720173
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG400.006050236554.374922
AGCGAAA2650.049.2554741
CGAAAGC3650.037.7396743
GTCTATA1251.2628061E-534.8071981
GCGAAAG3800.034.3420562
TCTATAC5500.027.681783
TATAGCT3201.2387318E-924.9218394
GTATACG1751.2534861E-424.8622861
GTATAAT2051.287546E-524.7612171
GTATATA3952.3646862E-1123.8656941
TAGACAG3702.6011548E-1023.5134815
GTATCAT3102.410161E-823.3919351
ATAGACA3803.619789E-1022.8947034
ATATAGC1902.1923624E-422.8947033
ATTATAC2252.650241E-522.5555213
CTATACC6150.022.3983424
ACTACGC2953.8713188E-722.118618
GTATAGA3355.4813427E-821.6462691
TATATAC2806.0864477E-620.7142563
GATTATA1850.00503949719.598651
Produced by FastQC (version 0.11.5)