FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1982-d10-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1982-d10-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861436
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG12990.1507947195148566No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG10070.11689783106348005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3950.051.3948751
GCGAAAG4650.043.6554762
CGAAAGC4900.041.4281583
GTACTGA6200.038.588326
AGGTACT6450.037.092654
TGATCCG800.00203655936.2496345
GGTACTG6550.035.4194955
GTATGGA7350.034.525471
GGTCGGA850.002741910334.1173066
ACTGATC7300.031.7805048
GCAGGTA7100.030.6334952
GTTACGT950.00472582530.5277821
GGATAGC7200.030.208035
TATGGAT7500.028.0330542
TACGGCT1306.482594E-427.8843364
GATCCGT1050.007708245427.618776
ATCCGTT1100.00967132526.3633737
TACTGAT9150.024.5625957
CTGATCC10850.023.3868649
TTAACGA1550.001808391923.3868622
Produced by FastQC (version 0.11.5)