FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1982-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1982-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820838
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG2800.038.8379635
GGACCGT750.001483479538.665356
GCATCGA3150.032.223087145
TAATACT5100.028.4304074
CGAAAGC4600.026.7925643
TCATACG1450.001226499124.9991512
CCATATG5950.024.368922
TCTATAC6000.024.1658443
TAAGGTC4600.023.64054
AAACGTT3702.6011548E-1023.5127126
ACGGTAG1550.001808572323.3863016
GCCTAAG5050.022.9695151
TATGCTG7650.022.7443245
AATATAC5450.022.613913
ATTATAG3055.339498E-721.3927143
ATATACT4751.2732926E-1121.3676934
ATAGTAG2053.6665096E-421.2187921
GGATGCG4801.4551915E-1121.1451137
ATACTAT2404.3628028E-521.1451136
GATGCGC5151.8189894E-1221.1157888
Produced by FastQC (version 0.11.5)