FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1982-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1982-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982023
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGC502.2686436E-886.999756145
AGCGAAA3750.052.202511
TATCGAC855.3415122E-542.646947
CTACGAG907.482466E-540.2776649
GCGAAAG5450.037.2476042
GGTCGTA800.002036625836.2498978
ATATCGA1051.8537224E-434.5237126
CGAAAGC6400.031.718663
CCTACGA1153.1621096E-431.521658
ATCGACG1153.1621096E-431.521658
CGGAGCG1658.41858E-526.3635649
ACCGTGC4850.025.41238
TATATCG1801.5202872E-424.16665
TATATAC5000.023.1999363
GTAATAT3803.6379788E-1022.895841
ATATAAC6800.022.3896433
GGGACGG2003.103042E-421.7499395
TATAGGC2003.103042E-421.7499397
GTCCTAC3406.427945E-821.3245541
TCCACCG4801.4551915E-1121.1457755
Produced by FastQC (version 0.11.5)