FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1982-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1982-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716233
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC11790.16461123684611015No Hit
ATTTTAGAGTGATCGCTTACACTGAAAAATATTTTGGCCTAAGTTTCATA8210.11462750250267721No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT7320.10220137860165618No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGA451.1937328E-464.44339
GTGATCG1600.054.3740279
AGTAGCG553.218667E-452.7263348
ACCGTCG450.00963870548.3324748
AGCGAAA2650.043.7728041
GCGAAAG3000.038.6659772
GAACCGT750.001483234638.6659776
GAGCGCG1051.8533866E-434.5231939
GTATAGC950.004726821630.5257723
CGAAAGC4000.028.9994833
TACCGAT1000.006073849728.9994837
TTACACT4100.028.292184
CCCTGTA3403.6379788E-1227.720095145
CAATACT3153.45608E-1127.6185554
TATAAGA4000.027.1870164
AGAGCGC1658.416315E-526.3631698
TTAGAGT4250.025.587784
TATAGAC1801.5198812E-424.1662373
GACGATG2151.865035E-523.60423145
TATAGTA1851.8301813E-423.5130942
Produced by FastQC (version 0.11.5)