FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1981_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1981_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080355
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC16340.15124658098495403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT1803.1996024E-936.2512471
GCACGCG1402.7470993E-531.0710628
CCGGTAA1402.7470993E-531.071062145
TACGTGT1652.3868815E-630.7572143
CCGACTA2054.0400664E-728.2923494
TATACTG4450.027.6963025
TACGGGG1100.0096724826.3633257
GTTACGT1958.752997E-626.0265391
AGCGAAA4500.025.7786671
TACACCG2559.488758E-825.5879335
TTACGTG2001.0652875E-525.3758752
GTTGACA9550.024.2940311
CTTAACG1550.001808220323.3879031
ATATAGT3803.6379788E-1022.8944664
GTATTAG2552.706609E-622.7458821
AGGTCGG5100.022.744838
GTATAAC3554.123649E-922.4655631
GTTATGT6200.022.2185081
CCATCGA2353.7098733E-521.595499
ACATGGT10750.021.5811425
Produced by FastQC (version 0.11.5)