FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1981-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1981-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854484
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA12900.1509683036780092No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG12390.144999789346553No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12100.14160592825611715No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT10620.12428553372561688No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT10380.12147682109904924No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA10180.11913622724357624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1800.052.3639641
TTACGTG1950.048.3331382
CGTGTTG2100.044.880775
TACGTGT2150.043.8370323
GCGATTA700.001056575841.428402145
GACGACT750.001483294838.6665084
TATACTG3450.035.7244955
GTTATGT7550.035.531741
ACGTGTT2900.032.499874
CAAGACG1851.6432023E-731.3512253
GTTGACA9800.031.0731221
GTAATAT2351.2605597E-930.8527471
CTGATCG950.004727008330.5261929
ACATGGT10050.030.2983865
TATGTGT9750.028.9998843
AGCGAAA2303.484638E-828.371111
GCTACTA2303.486639E-828.3694529
GTATTAT10750.028.3271241
TGTGTTG10900.027.9356655
ATACTGT4450.027.6965186
Produced by FastQC (version 0.11.5)