FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1981-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1981-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652003
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC10280.1576679861902476No Hit
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC8370.12837364245256538No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT7480.11472339851197003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT700.093.221391
TTACGTG1009.094947E-1265.249962
GTGATCG753.759178E-757.9999739
GGATAGC2450.041.428555
AGCGAAA2150.040.4681971
ACATCGT750.00148301138.666656
GCGAAAG2650.038.3048061
CCGACTA800.002036101936.249984
GGTACTG2650.035.5660175
TACGTGT1905.4205884E-934.3420873
ATACAGC5450.033.2568636
CGAAAGC3100.032.741922
GGACCTA1551.4639772E-632.741926
GTATTAT6100.032.0926061
AGGTACT2950.031.9491354
ACGTGTT2051.1374141E-831.829254
CTACTGA5250.031.7618896
GTTATGT2558.731149E-1131.2768921
CGTGTTG2101.4382749E-831.0714115
TGGATAG3750.030.9333174
Produced by FastQC (version 0.11.5)