FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1981-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1981-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708641
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTT350.003566267462.142013
ATAACGA553.2185766E-452.7265553
GCGTTTA2100.048.3326761
TATACGG450.00963850448.3326765
TAGTACT2101.4388206E-831.0710074
CTAGTAC1453.489299E-529.999593
AGCGAAA4350.028.3329471
GTATTAG2054.0368286E-728.2922971
GCGAAAG5700.027.9820771
CGTTTAC3950.027.531272
TAACTAT1358.0810196E-426.8514846
TATAACG1100.0096703626.3632772
CGAAAGC5300.025.9902132
CTTACGG2559.4792995E-825.5878873
TACGGAG2651.3753197E-724.6223075
TAGGTAG3952.3646862E-1123.8604345
CCTTACG3102.4157998E-823.3867782
TTACGGA3152.8625436E-823.0155584
GCATAAC2252.6504125E-522.5552486
ATAAGAC1952.6139428E-422.3073883
Produced by FastQC (version 0.11.5)