FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1981-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1981-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509581
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC8010.15718796422943554No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA5200.10204462097291697No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1551.4551915E-1146.7741931
GTACGGT657.3331816E-444.6153871
TATACTG3700.037.2297335
GTATAGT1656.006849E-835.1515161
TTATACT3350.034.6268654
ACATGGT2950.034.406785
GTTGACA3100.032.7419361
GAATAAC1851.6409285E-731.3513531
AAGGTCG1652.3833963E-630.7575769
GTATTAC1204.05528E-430.2083341
TAATAGT1504.3945274E-529.04
GCGAAAG2603.7107384E-927.8846172
GTCTATA1050.007704961627.6190471
CGAAAGC2808.176357E-925.8928593
GCAGTAC1409.987843E-425.8928598
GTTATAG1409.987843E-425.8928591
GTCTAAT1701.0303206E-425.5882341
TAATACA4550.025.4945074
TGACATG4850.025.412373
TATGTAC2051.2886487E-524.7560983
Produced by FastQC (version 0.11.5)