FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1980-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1980-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919059
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG12720.13840243118232887No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG10600.1153353593186074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACTA1553.470268E-837.418899
GTTACGT1752.4247129E-937.293371
TTACGTG1803.1977834E-936.2515262
GTTATGT8800.032.961311
TATACTG3100.032.741535
GACGACT900.00362829932.2218254
TAGCATG11250.031.5791032
GTTGACA8750.030.6634371
GCGGACC950.004727384530.5259389
ACATGGT8200.030.0606045
CTACTGA11100.029.391536
TAGTACT1753.774543E-628.9996414
TACGTGT2054.0389932E-728.2923343
CATGTTG10950.027.1457825
TACTGAA12950.026.8722537
GCATGTT12950.026.8722534
GTAGCAT15800.026.1604331
GACGATG1958.750625E-626.026735145
TTGATTA6400.024.922922145
GTAACGG1751.253869E-424.8622461
Produced by FastQC (version 0.11.5)