FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1980-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1980-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581168
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC8540.1469454615532858No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT7950.13679349172700495No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG6620.11390854279657518No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC6060.1042727748258679No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG2450.044.3865975
GTACACC1859.640644E-1139.1881683
GTAGGCT1701.8262654E-938.381356
GTATACT800.002036175736.2490544
CCTATAT1251.2644037E-534.799091
GCATTCG1051.8530474E-434.522911
ACCGTTA900.003627454632.2213828
GTATAAT1255.152003E-428.9992431
GGCTCCG2303.4833647E-828.3688269
CTATGCG1555.4974917E-528.0637864
ACCGTAG1050.007706819627.6183288
CACCGTA1050.007706819627.6183287
TAGTACT1050.007706819627.6183284
TATAACC1907.1501363E-626.709833
GGTTACA1100.00966954826.3629461
TAGTATA1100.00966954826.3629462
ATACTGT4350.024.9993466
GTAATAT2351.3291392E-624.6802061
TAGAGTG2101.5539268E-524.1660375
GTGTAGG3601.8371793E-1024.1660371
Produced by FastQC (version 0.11.5)