FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1980-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1980-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734024
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG64670.8810338626529922No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG63400.8637319760661778No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC60500.8242237311041601No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA57950.7894837226030756No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGATCCAAA48070.6548832190773054No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT41200.5612895491155603No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG39760.5416716619620068No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA36990.5039344762569071No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT36540.4978038865214217No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA33960.4626551720379716No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG25860.3523045567992327No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC23600.32151537279435005No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC22400.3051671334997221No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA20590.2805085392303249No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC20450.2786012446459516No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG20410.2780563033361307No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA19290.2627979466611446No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT19210.2617080640415027No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA18430.25108170849999456No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG17940.24440617745468815No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG17250.23500593986027704No Hit
GATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGA16800.22887535012479157No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC16310.22219981907948516No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC15300.2084400510065066No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA15140.20626028576722286No Hit
CTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGATCCAAATG14360.19563393022571468No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC14260.19427157695116234No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT14200.19345416498643098No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG14010.19086569376478155No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA13940.18991204647259488No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA13840.18854969319804257No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA13820.1882772225431321No Hit
AAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCATCAT13650.18596122197639314No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG13480.1836452214096542No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG13330.1816016914978257No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA13120.1787407496212658No Hit
GATATGAACTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGG12750.17370004250542218No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG12570.17124780661122796No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA12560.17111157128377275No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG12380.16865933538957853No Hit
TTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATG12210.16634333482283958No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA12090.1647085108933768No Hit
TTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATA12050.16416356958355585No Hit
CTGCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCAT11800.16075768639717503No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG11720.15966780377753315No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG11610.1581692151755256No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT11570.15762427386570466No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT11250.1532647433871372No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT11200.15258356674986104No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC11110.15135744880276394No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA10940.149041448236025No Hit
CCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACAG10860.14795156561638312No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA10830.1475428596340174No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT10830.1475428596340174No Hit
AAATAGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTT10810.14727038897910694No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT10740.14631674168692033No Hit
CATCAATATTCAGAAAAAGGGAGGTGGACAACAAACACAGAGACCGGAGC10620.14468191775745753No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA10570.14400074112018135No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG10570.14400074112018135No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA10510.14318332915544996No Hit
GCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTGTT10370.1412760345710767No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA10140.13814262203960634No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT10100.1375976807297854No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT9970.13582662147286736No Hit
CAACAGAACACATCAATATTCAGAAAAAGGGAGGTGGACAACAAACACAG9860.13432803287085981No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT9800.13351062090612842No Hit
CCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAGGGAAG9670.13173956164921038No Hit
AAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTT9620.1310583850119342No Hit
GCTTCATGTATTCAGACTTCCATTTCATTGATCCAAATGCATTGTTGAAG9610.130922149684479No Hit
CTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATA9550.13010473771974757No Hit
GTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCC9550.13010473771974757No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG9530.12983226706483714No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTTGCAGATGC9470.1290148551001057No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT9340.12724379584318768No Hit
CCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCAG9220.1256089719137249No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC9100.12397414798426208No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG9020.12288426536462022No Hit
TTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGTC8940.12179438274497836No Hit
CCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTG8910.12138567676261267No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA8820.12015955881551556No Hit
CTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGT8780.11961461750569463No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT8770.11947838217823942No Hit
GTTGAAGCACAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACA8630.11757108759386614No Hit
GTGCAGCACTTCTGGTACATTTGTTCATCCTCAAGAATTCCCCTTTGGCT8600.11716238161150044No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC8520.11607249899185858No Hit
GAATATAGGCTGTTGAACACAGATTTTGCTAGTAAGGTTCTGCACACCTT8490.1156637930094929No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT8470.11539132235458241No Hit
CCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGGGAGGTGG8450.11511885169967195No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG8360.11389273375257486No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG8320.11334779244275392No Hit
GATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTC8320.11334779244275392No Hit
GTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAAT8300.11307532178784345No Hit
TGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTT8280.112802851132933No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA8270.11266661580547775No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT8270.11266661580547775No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG8200.11171296851329113No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC8140.11089555654855972No Hit
CCCCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAA8110.11048685056619402No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG7970.10857955598182074No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCAT7860.1070809673798132No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG7780.10599108476017133No Hit
GATCCAAATGCATTGTTGAAGCACAAGGTGTGCAGAACCTTACTAGCAAA7780.10599108476017133No Hit
GGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAG7780.10599108476017133No Hit
CCCCAACTCAACCCTATTGATGGACCATTACCTGAAGACAATGAGCCGAG7740.10544614345035039No Hit
CAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA7610.10367508419343235No Hit
CCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACA7610.10367508419343235No Hit
CTTCCATTTCATTGATCCAAATGCATTGTTGAAGCACAAGGTGTGCAGAA7600.10353884886597714No Hit
GGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCC7540.10272143690124573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA7850.0122.842072
GGTACTG8600.0112.9721765
AGTAGAA9400.0109.5430451
TCTATAA203.864682E-4108.756794
CAGGTAC9650.0100.679863
GGTCAAA3000.099.103031
AAATATA3400.087.431945
CTATAAC259.3834155E-487.005445
GTACTGA11750.084.53726
AGCAGGT12200.079.05261
AGGTACT13900.073.547764
CTGATCC13450.072.7691049
GTCAAAT4100.072.504532
ACTGATC15100.070.0989468
AGAAACA15200.069.1655044
TTTGGAC15250.065.610663
TAGAAAC15950.065.458633
GACAGTA15450.064.287667
ACAAGGC11400.062.9601758
CCGGGTT350.003565243262.146744
Produced by FastQC (version 0.11.5)