FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1980-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1980-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749907
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG16710.22282763062619768No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC12620.16828753432092247No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC11850.15801959442970928No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT8500.11334738840949611No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA7900.10534639628647287No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG604.944454E-448.3339
TATACTA2400.039.2731822
TCGCCAT7600.038.15763145
TATAGCG1354.9668415E-737.594842
TATACTG5300.036.9361725
CTATTAG8650.035.2091141
TATTAGT8650.034.3662152
TTATACT4750.033.5809564
GTATAGG6400.032.8579061
TAACTAT1851.6399099E-731.3574071
CTACTAG2351.2605597E-930.852916
CCTATAG3300.030.7594153
GTCGGTA4550.030.274517145
GCGGTAT1703.0089886E-629.8547276
ATTAGTA9750.029.7453693
GTATAAC1504.3914697E-529.0056021
CTATAGG4050.028.6475091
CCTTATA5650.028.2317772
TATACCG4900.028.1139265
GTATAAT1306.4741104E-427.8900011
Produced by FastQC (version 0.11.5)