FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1979-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1979-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930508
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG5050.034.4570665
GCGAAAG3750.032.8699842
TGACGCG900.003627266732.223745
GTGATCG1402.7455886E-531.0728919
AGCGAAA4100.030.0672381
ATTACGC1050.00770438827.6218323
GTTACGT1100.00966143426.3691271
GGTTATA3607.2759576E-1226.1860081
ATAGGAC2751.9664003E-723.7296663
CGAAAGC5650.023.0996743
GATATAC2903.2823482E-722.5022683
GTATAAG3355.4857082E-821.64631
ATACAGC7050.021.5967626
GTAATAT5101.8189894E-1221.327971
TAGGTAC3106.2401887E-721.0493774
GTTGACA7600.020.9912131
GTATATC2908.233643E-620.0041661
ATAAGAC3701.5678597E-719.5965713
CAATAGG3751.8038736E-719.337361
CCTATAC2256.862529E-419.3352813
Produced by FastQC (version 0.11.5)