FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1979-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1979-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071979
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG657.333808E-444.6191064
GCGAAAG5500.036.9138871
CTAGTAC3153.45608E-1127.621353
CGAAAGC7050.026.7398152
GTTACGT1658.4116866E-526.3670641
TATACTG5000.023.2019335
AGCGAAA4700.023.1413041
GTATATA6750.020.4100591
GTATTAC5052.910383E-1120.1016241
TTACGTG2205.900431E-419.7743762
GTACTAG1850.005043317619.5971411
TAGTACT3751.8068931E-719.3349464
GTATAAA10150.018.5738431
TAGTACC7100.018.3818134
TAGGACT9150.018.2255614
ATGCCCG2000.00791689318.12482145
GTAGCCG2050.00912734817.6827499
GTAATAT4959.740688E-917.5780431
CTAATAC2952.1245585E-417.2048233
TAATACT5501.9281288E-917.1377934
Produced by FastQC (version 0.11.5)