FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1979-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1979-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871783
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAG855.3410156E-542.6469423
GTGATCG1105.263624E-639.545359
GTTACGT1303.6977144E-739.0383531
AGACGAC850.00274183134.1175543
AGTCGAA1000.00607411828.9999182
GACGACT1050.007708023327.618974
TTACGTG1855.81514E-627.4323582
GTTGACA7900.026.6138481
AGCGAAA3005.857146E-1026.583261
AAGTCGA1100.00967104626.3635651
GCGAAAG4200.025.8927841
TATACTG5350.025.7475935
GGGTTAC1450.001226336724.9999311
GCTACTA2401.5977275E-624.16669
GTAGCAT11450.024.061071
GTTATGT6300.023.015811
CTATACA2552.706487E-622.7450354
GTCTAAC1600.002174962522.6561871
TAGCATG11000.022.4090272
ACATGGT9500.022.131525
Produced by FastQC (version 0.11.5)