FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1978_d08_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1978_d08_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4300
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAATGTATTCATCACT70.16279069767441862No Hit
GCTTAGGACCCTATGGCTCATGTCTCCTAACATCCCTCTTCTAGGAACAG70.16279069767441862No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT70.16279069767441862No Hit
GAAAAGAGGAGAGAGGAATGGAGTCAGCCCGTGAGGTGGGGGGATTGCAC60.13953488372093023No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC60.13953488372093023No Hit
GGTAGTATGTAGGCCATTTCCCAGTAGTGTGACCCCACTTTCCATAGCTG60.13953488372093023No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT50.11627906976744186No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA50.11627906976744186No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT50.11627906976744186No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC50.11627906976744186No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG50.11627906976744186No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA50.11627906976744186No Hit
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC50.11627906976744186No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)