FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1978_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1978_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8500
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC240.2823529411764706No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA210.24705882352941178No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG190.2235294117647059No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG160.18823529411764706No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT160.18823529411764706No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG160.18823529411764706No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC140.16470588235294117No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC130.15294117647058825No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG130.15294117647058825No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT120.1411764705882353No Hit
CCGCTATATGATGCGATAAAATGCATGAAAACATTCTTCGGCTGGAGAGA120.1411764705882353No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC110.12941176470588237No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG110.12941176470588237No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA110.12941176470588237No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA110.12941176470588237No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA100.1176470588235294No Hit
GTACATAATGAAGGGGGTTTACATAAATACAGCTTTGCTCAATGCATCTT100.1176470588235294No Hit
GTTGAGAATGGAGCGATTTCTCTTGGGAATCCATGAATGTGTAGTTGCAA100.1176470588235294No Hit
ACACAATTACCACCTTTTAAATCAGTCTTCAGGATTCTTCCATGGCTCCC100.1176470588235294No Hit
ATTATGTACTCAGTGGCCCTGCAATGAGACACTTCCGCTGTGAAGTAGTT90.10588235294117646No Hit
CCTTTTAAATCAGTCTTCAGGATTCTTCCATGGCTCCCTCCTGAAAGAAC90.10588235294117646No Hit
TGTCCATACCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGT90.10588235294117646No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC90.10588235294117646No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCATTCTGCTGT90.10588235294117646No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT90.10588235294117646No Hit
CCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCACAT90.10588235294117646No Hit
CAATATCACTAATAACTATACTACTAGTAGTAACAGCAAGCAATGCAGAT90.10588235294117646No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT90.10588235294117646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAA259.0675376E-487.0145
AACCAGG259.0675376E-487.08
AAAACCA259.0675376E-487.06
ACCAGGC259.0675376E-487.09
GTACTAA350.003446095962.1428571
CTAAAAC350.003446095962.1428574
TACTAAA350.003446095962.1428572
AAACCAG400.005847347454.3749967
ACTAAAA400.005847347454.3749963
TAAAACC400.005847347454.3749965
TGATTTA200.00601012229.0105-109
GATTTAA200.00601012229.0105-109
CTGATTT300.001470909524.166668105-109
Produced by FastQC (version 0.11.5)