FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1978_d02_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1978_d02_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2968
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTATGGCTCATGTCTCCTAACATCCCTCTTCTAGGAACAGGATGATTT50.1684636118598383No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC40.13477088948787064No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC40.13477088948787064No Hit
GATATGTTCCTTCTATTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCC30.10107816711590296No Hit
CCATTTATGAGCTGTGTGCCCCATGGCAACTGGCTCAGCTTTCTGAGCCT30.10107816711590296No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT30.10107816711590296No Hit
GTCTGACAGCCTGTTGTAGTTCAGGCGAATGAAGGCAAGGTTGGGGAAAC30.10107816711590296No Hit
TCCTAGCACTGTGGGTATGCTGGCTGCACGCCCAGCGATGTGTGATTCTG30.10107816711590296No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAA30.10107816711590296No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA30.10107816711590296No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA30.10107816711590296No Hit
GGGTAACCTTTGCCAGGTGATACCTGATGAGATTTGTCACCCCAGGGCTT30.10107816711590296No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG30.10107816711590296No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG30.10107816711590296No Hit
GTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGTAATTGATTT30.10107816711590296No Hit
CTTCATAACGGAGCTCCCCGTGGAGTCCTTCCAGAATGCCACCGGCCTGA30.10107816711590296No Hit
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC30.10107816711590296No Hit
CCACAGAACTGGGGGCACAGTCTGAAAACAACTGAATTGTGGGATCTCTA30.10107816711590296No Hit
GTCCCTGTCATCCCCCCACGCATCCATTACCTCTACCTGCAGAACAACTT30.10107816711590296No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGCAC100.0067389184145.03
ACACAGC100.0067389184145.08
AAAAGGG100.0067389184145.05
CACAGCA100.0067389184145.09
Produced by FastQC (version 0.11.5)