FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1978-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1978-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536448
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA400.006049362554.3759
TAGACCG553.217654E-452.7272765
TACACCG1002.73176E-643.55
CGAAAGC2600.036.252
GTACACC1153.1601594E-431.521744
GCGAAAG3100.030.4032251
GTATTAC2756.7502697E-926.3636341
AGCGAAA2701.6458944E-724.1666681
CGTAGAC1801.5190041E-424.1666663
TATTAGG1902.1913281E-422.8947372
GTATAGT1650.002599303421.9696961
GTATTAG3351.312088E-619.4776131
CTATACT2256.8619975E-419.3333344
GTCTAGA3001.10395995E-519.3333341
ACCATGC4954.7293724E-1019.0404038
CTATTAA4202.849265E-818.9880941
TAACACC2307.955368E-418.9130444
ATAGGGC1950.00683652118.5897453
TATAATA3902.7256465E-718.5897452
ACTATAC2359.192495E-418.510643
Produced by FastQC (version 0.11.5)