FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1978-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1978-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873581
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3400.042.6518251
GCGAAAG3750.038.668772
GTTACGT1851.6416016E-731.3548551
TTACGTG1851.6424383E-731.353062
CGTGTTG2101.4386387E-831.0731185
CGAAAGC5200.027.8861353
TACGTGT2156.12019E-726.9782123
TATACTG4750.024.4223845
GTATAAC3102.4141627E-823.389711
AATACGT1550.00180833623.3870327
ACGTGTT3004.5511842E-721.7511844
TATGGAC3757.754352E-921.2678243
GTATAAA6600.020.8735431
GAGCGAA1750.003655997820.71661
TTATACT4752.728484E-1019.8431874
GTTGGCC8200.019.4511668
GTACCTA5707.2759576E-1219.0799876
GTTATAC2701.07852036E-418.797323
ATAGATC2801.4258496E-418.124958
TATACTA2901.8642537E-417.5009545
Produced by FastQC (version 0.11.5)