FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1978-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1978-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335680
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG4000.059.8144342
CGAAAGC5300.043.7749983
ATAGGCG700.00105660141.429914
TGCGTTA900.003628756632.2220548
CTCGTAC1000.006074218529.0009371
AGCGAAA8150.028.4671761
ACACGTC1907.158076E-626.7103888
GCGTTAA1100.00967296826.36359
TACTACG2507.835297E-826.0998677
GTCCTAT11200.025.246351
GTATATA7350.024.6606621
TATAGGC1550.0018088623.3869767
ACTACGC3152.8670911E-823.0157558
CCTATAC2552.7074548E-622.7458323
GATTCCG1600.002175595422.6561328
TATACTG5900.022.1193585
GTTATAC5350.021.6829433
GGGTTGA12950.019.5952266
TTTGCGA1900.00588907619.0788488
GTCTAGG8400.018.9887081
Produced by FastQC (version 0.11.5)