FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1978-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1978-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824940
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATGGGTGTGTGTAGACCCTTAAAAAATGTCACAGGCCCTGTGTGTC12630.15310204378500256No Hit
GTGTAGACCCTTAAAAAATGTCACAGGCCCTGTGTGTCGCGAATATGTTG10160.12316047227677164No Hit
GTTATAGAGCCTACTAATCCCCAAGGCAAAGAACTGGACTGTGATGCCCT9390.11382646010618955No Hit
GGGCTGAACCGTGCACAGTGTCTTATGGGTTGAGATTGGCTAACTTATAA9350.11334157635706839No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC8830.10703808761849345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA502.3035063E-672.512581
AGCGAAA2500.055.109561
ATATACG1453.8380676E-1044.9996224
GCGAAAG3550.042.8895652
GAACCGT4850.037.3708236
CGAAAGC4350.036.666363
CCTATAC2302.7284841E-1134.6757242
GTTTCGC850.002741820434.117363145
AGACGGT1707.820563E-834.117363145
TATAACA3450.033.6229064
GTATAAG1601.8740502E-631.7242551
CTATATA3900.031.6042182
GTGAGTC4000.030.8141129
ATAGATC950.004727013430.5260643
TGAACCG5950.030.461935
CTGATCG1204.0561942E-430.2099119
AGGACGT1453.4898614E-529.999755
TCGCTCA1703.0096453E-629.8545029
TATACGG1952.6065572E-729.743345
ACCGTGC7600.029.5739158
Produced by FastQC (version 0.11.5)