FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1977-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1977-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1217448
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12460.10234523363626209No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC450.00963669348.337073
GTTATGT7950.041.9563751
GCGAAAG4750.039.6889082
GTTACGT2200.039.551761
GTGATCG2750.034.272569
AGCGAAA5800.033.7553831
CGAAAGC5900.033.1805343
CCGACTA3800.032.4367184
CGTGTTG2901.2732926E-1130.002325
TTACGTG3102.7284841E-1128.067842
AACGGCG1050.007709281527.618914145
TATACTG5800.027.5021275
TTCCGGC1100.00967068526.3645947
GTTGACA9700.025.4164241
ACGTGTT3702.6011548E-1023.5153334
GTTGGCC18850.023.4623898
CCACCGA5650.023.1010281
AGCACCG6300.023.0176535
TGTTGGC18950.022.573387
CTACTGA14500.022.501746
Produced by FastQC (version 0.11.5)