FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1977-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1977-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904236
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12050.1332616706258101No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG11120.1229767450090463No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA10910.12065434245042224No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC9560.10572461171641033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTG1800.076.531842
GTTACGT1950.070.644771
CGTGTTG2250.064.447865
TACGTGT2750.050.0935673
GCGAAAG3500.035.2161521
GTTGACA8850.032.77011
ACGTGTT4350.031.6683444
CGAAAGC3900.031.6042392
CATTCTA10650.030.635432
GTTATGT6950.030.2534031
CCGACTA4350.030.001594
GTGATCG1453.490084E-529.999939
CTACTGA13550.029.9646876
TACTGAA14450.029.6020097
CAACCCG2701.72804E-1029.53697145
ACATGGT9300.028.8456155
AGGTCGG3351.8189894E-1228.1342648
GCGATTA1306.482553E-427.884552145
TGTTGGC13600.027.7205247
GTTCGAT2456.4372216E-826.6325919
Produced by FastQC (version 0.11.5)