FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1977-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1977-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023755
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT10710.10461487367583065No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG10350.10109840733378592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1600.063.4404681
TTACGTG1850.054.867432
GTGATCG1351.9099389E-1048.3332379
AAGGTCG2400.039.2707569
TATACTG8500.033.2662665
AGCGAAA5100.031.2759721
CGTGTTG3300.030.7590165
GTTGACA8800.029.6604791
GACAGTA3950.029.3670317
GGTACTG4700.029.3098835
GTTATGT7150.028.3929391
TTATACT7800.027.885924
GTATTAG2354.2959073E-827.7672581
AGTCCGC3153.45608E-1127.618994145
GGACGGC3503.6379788E-1226.9298322
GTATAGG2705.5606506E-926.853111
CCGTAGA1907.1530503E-626.7117751
CAGTATG3800.026.7104729
TACGTGT3850.026.3648723
GCGAAAG6350.026.2610722
Produced by FastQC (version 0.11.5)