FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1976-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1976-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403204
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTT502.3038574E-672.499387
CATACCG651.0917205E-555.7756735
CTATAGT400.0060366254.4015161
AGCGAAA2050.049.5363461
GCTAGAC1109.930409E-846.141693
ATACCGT951.02710066E-438.16236
ACCGTTG1001.3900467E-436.249698
CATGGCG850.00274016934.1173559
AAGCCCG1551.4622328E-632.741657145
CTAGACC2008.913048E-932.628774
GCGAAAG2950.031.9568062
TGCGATC1153.158937E-431.5214678
ATACACC1851.6378362E-731.354974
CGTGATC1204.0539974E-430.2080758
GTATAAC1453.4736982E-530.0146331
GTATGTA1702.9944385E-629.86751
TAGAGTG1753.763882E-629.003355
CCTATAC1255.1449734E-429.003353
TAGACCC2354.2793545E-827.7691615
GTGCTAG1050.007684772427.632521
Produced by FastQC (version 0.11.5)