FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1976-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1976-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901131
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG3650.043.6983342
CGAAAGC4250.035.8232843
TAGCTAG850.00274193534.1174139
TTAAACG2151.8102583E-830.3486259
TATAGCG1453.490181E-529.9997925
AGCGAAA5100.029.8527361
GTTATCG1000.00607434628.9998049
TACATCG1504.398437E-528.99985
ATAGCGG1555.500084E-528.0643236
GTACGAG1050.001290771927.6188561
ATAATAC5250.026.2379153
TAATACT4550.023.9009344
TATAGGT2151.8655715E-523.6044867
AATACTG8350.023.4429515
GAACTGT9400.023.1381386
ACATCGT1902.1930377E-422.8945796
GTACTAC1650.002600765621.9695459
ATAGGTC4358.367351E-1121.6665178
TACTATA3809.040377E-920.9866982
TCTATAC4151.0913936E-920.963713
Produced by FastQC (version 0.11.5)