FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1976-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1976-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874489
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG755.638867E-1177.341842
GCGAAAG3550.051.0619542
GCGGTAT1203.61797E-948.3358886
CGAAAGC4000.045.3148963
AGCGAAA4550.038.2525331
CGGTATC1458.697061E-734.9998477
ATAGCGG1501.1332068E-633.835123
TAGCGGT1751.0084841E-733.1446084
TACTCGT950.004727075330.5261827
TAAACCG1000.006072478429.0015325
GTATACC1701.0291761E-425.5954421
GTACAGT2601.1438169E-725.0974796
AGCGGTA2051.2894956E-524.7574045
AAGGTCG1851.8304643E-423.5134129
TATACTG7300.022.8436745
TATACTA3253.9863153E-822.3088725
ATGAGCG1650.002600666421.9696039
CATATAC3851.0501026E-820.7153823
GTATAGG2857.079014E-620.356611
AGGGTGT7500.020.3010736
Produced by FastQC (version 0.11.5)