FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1976-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1976-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766264
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT13700.17878955555787562No Hit
CCGCTATATGATGCGATAAAATGCATGAAAACATTCTTCGGCTGGAGAGA8140.10622970673292756No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCG100.007090656144.99922145
AGCGAAA1400.0119.13761
GTGATCG1700.072.499619
GGCTAGC2600.069.729361
TATACGG1009.094947E-1265.249655
TACGGGG902.8194336E-1064.44417
ATATACG902.8194336E-1064.44414
GCGAAAG2650.062.928292
GACGTCT400.00605047854.3747068
TCGACAC553.218612E-452.726993
GCTAGCA3450.052.539372
TTAGAGT2550.051.1761974
TAGCGGT5100.049.7546354
AGCGGTA5150.049.271585
GCATTAC604.9381226E-448.345691
CTAGTAC450.00963848948.3330733
AGACGGT752.5501024E-548.333076
ATAGCGG5600.046.606893
TATAGCG5700.045.7922132
GAGTGAT3250.044.6151437
Produced by FastQC (version 0.11.5)