FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1975_d08_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1975_d08_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC34.6875No Hit
AGTTTGAATTTGTGTATCACCTCTGTGGCATCGGTAGGTGTATCTGTAGT11.5625No Hit
ATGATAAACAACGACCTTGGACCAGCAACAGCCCAGATGGCTCTTCAGCT11.5625No Hit
ATTACATACAAATGTTGCATCTGCAAGATCCATTGGACATGGCCCAGAAC11.5625No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC11.5625No Hit
CTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCAT11.5625No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA11.5625No Hit
GCAGGGGGGAGCGAAAGCAGGTTCGGATGCTCGAACTGCGAATGCCTTGT11.5625No Hit
GATTCTGGAGGAAGAATCTGACGAGGCACTTAAAATGACTATCGCTTCAG11.5625No Hit
GATGTTAGATTATCAATGGGGGCACTTCTGAACAGGTATCATTATTGTTC11.5625No Hit
GAGCATTCTTTTCGTGTGGGGCATACATCACCCACCCACCTATACCGAGC11.5625No Hit
GAACAGGAACAGGATACACCATGGACACAGTCAACAGAACACATCAATAT11.5625No Hit
AATTGAAGGAAGAGACCGAGCAATGGCCTGGACAGTGGTGAATAGCATCT11.5625No Hit
CAAAAAACCTCCGACCTTTCAGAAGATTCTGAACTTCGGGAGGCAACGCA11.5625No Hit
CATTTGGTCTAATAAGACTGAAGACCTGACTGTTCTGAAGCAGACGGAAA11.5625No Hit
ATTAAGGACAGGTCCTACCTGGGCCACGAGAAACACCGACGCAAGCTGAG11.5625No Hit
GTTCATAGGGTTACACAGTCTTCCCTGGTAATCTTCATCCATCAACTCCC11.5625No Hit
ATTCCAACTGGCCTCCGATATGAACTGCTAGGGAAGAATTTCTCGAATAG11.5625No Hit
ATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGTCGATTAAAAATCA11.5625No Hit
ATGCTGTTGAACCCCACCAAAGATGGGAGTTTCTACAAAGATGAGAAAAG11.5625No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCCAATGA11.5625No Hit
AGGCACGATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCGAAGACTCCCC11.5625No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT11.5625No Hit
CTATAGGACTTGCAAGCTAGTTGGAATCAACATGAGCAAAAAGAAGTCTT11.5625No Hit
CCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTG11.5625No Hit
ATGCATATCTTTTGTCTTCTTTGCCGAGGATTAGAAATCCTCTCAATACT11.5625No Hit
GAAAGCCTGATGTGGGACTCGATCCCAGGCCCCCGGGATCATGACCTGAG11.5625No Hit
GTGCTAAGTGGTGGAGAAGCCTCTGGAGAGACGCAGCTCGGAGAAGACTG11.5625No Hit
CCTCATGTCAGATGTTCTGCCCTCGGTATTCCCCTTAAACGCAGCCATAA11.5625No Hit
CAATTGTTCTCTCCACTTTCCGTTTCTGTTTTGGAGGGAGTGGAGATCTC11.5625No Hit
GCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCG11.5625No Hit
ATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAGACACA11.5625No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG11.5625No Hit
GCAGGGAAGAATTGATTATTATTGGTCGGTACTAAAACCAGGCCAAACAT11.5625No Hit
CTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGA11.5625No Hit
GGTTTGAAGGTCCTATTCAAATCTTCTGTTGTCACGCTTGTTGTTGTGTC11.5625No Hit
CCTCCTGTGAGGCCTCGTGTGGTTTGGGGCTGGCGCCTCTACGTGTGGGC11.5625No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG11.5625No Hit
CCACTCTACACAGCACATTTAAAAATACAGAGTGCTAACGTTGGCACAGC11.5625No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG11.5625No Hit
CTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTA11.5625No Hit
AACTGATAGTCAGCGGAAGGGACGAGCAATCAATTGCTGAGGCAATTATT11.5625No Hit
ATGCCCATACGTCTTGTATTTGGTCATCAATCTTATTATTGATCATATTG11.5625No Hit
ATCTTCCTCAGGGTATTGTGGGCCAGGTTGAGCTGCATGAGGTTCTTGAG11.5625No Hit
CCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGTTTGGCCTG11.5625No Hit
GGGTGGGATCTGATAGGACCGTGTTCATCGTTGAGTGTTTCTGGTATCAG11.5625No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT11.5625No Hit
ATATTTGGGTGACAAGAGAACCTTATGTATCGTGCGGTCTTGGTAAATGT11.5625No Hit
CTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTT11.5625No Hit
AAAGCAGGGGGAGTGGGAGAGGGGAAACAGGTTTCCCTCCAAGCAGGAAG11.5625No Hit
GTATAGCTCGAAACAGCCTTTCCCATCTTCCATAGCATTGGAGCCCAGTG11.5625No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT11.5625No Hit
GTGGATCATATGGCCATAATTAAGAAGTACACAACAGGAAGAAGTATTGA11.5625No Hit
ACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGG11.5625No Hit
GTTGGTTTCAGATGGAGGGCCGAATTTATACAACATCCGGAATCTTCACA11.5625No Hit
GATATGAACTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGG11.5625No Hit
CTACTAATAACCCGCCTTGTTTCTACTAATAACCCGCCTTGTTTCTACTA11.5625No Hit
CCCAAGGAACAATGGGCTCCAGGCTAAGAGCTGCCTAGGGACCCCTTCCA11.5625No Hit
CATTTGAATTGAAGAAGCTGTGGGAGCAGACCCGCTCAAAGGCAGGACTG11.5625No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACT11.5625No Hit
GACATTGAGTGACTAAACACCTCCAAGGTGGTGATCATTCTGTCGGCCTG11.5625No Hit
CCATATAGCCATGGAACAGGAACAGGATAAGTGCTGCACTCTATTCGAGA11.5625No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATGC50.0145.06
ACATGCA50.0145.07
ACACATG50.0145.05
GACACAT50.0145.04
GAACTCA50.0145.0145
ATGCACT50.0145.09
CTAGACA50.0145.01
AGACACA50.0145.03
TAGACAC50.0145.02
CATGCAC50.0145.08
Produced by FastQC (version 0.11.5)