FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1975_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1975_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819409
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT951.9197214E-645.7920156
TCTATAC4500.040.2800143
TATACTG2600.039.040635
CGAAAGC4700.038.565973
CCCTACG1102.434973E-432.9563752
TAAACCG2455.4569682E-1132.552835
GTATACG900.003626864432.2240141
CTATACC4800.031.7205124
TACGAGG1153.1605744E-431.523494
GGATGCG6100.030.9014727
GATGCGC5900.030.720178
TATACCA7100.029.614325
CTACGGT1000.00607217629.0016144
CATATGG7600.027.666012
CCTACGG1050.00770556427.620583
TCTATAG2104.9821756E-727.620583
GACGATC1050.007707859427.6188969
CTAATAC4050.026.8533423
TATACAG5150.026.7490585
AATACTG5450.026.6069815
Produced by FastQC (version 0.11.5)