FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1975_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1975_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977943
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGC604.9440895E-448.33519145
AGCGAAA3000.038.6721081
ATAACCG1000.00607173429.0025985
CCTATAG2853.2196112E-1027.9849623
GCGAAAG4450.026.0697482
GTATTAC3900.026.0293051
CCATCGA2259.108171E-725.7774529
ACCGTGC4400.024.7155958
GTAATAT2701.6465492E-724.1700671
GTCGCGG1500.001493915224.167595145
CCTATAC4154.3655746E-1122.712883
CGTATAA1600.002175276222.6559628
ATATAGG2603.2001335E-622.3108311
CGAAAGC5200.022.309693
GTCCTAT5900.022.1217581
TATACTG5700.021.6247435
GTCATCG3055.340571E-721.3931718
TATTACG1700.0030911421.325442
CTATAGT2455.1103292E-520.7161434
TTACGTT1750.003656774820.7161434
Produced by FastQC (version 0.11.5)