FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1975_d02_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1975_d02_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714355
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC11710.16392409936236185No Hit
ATTTTAGAGTGATCGCTTACACTGAAAAATATTTTGGCCTAAGTTTCATA8160.1142289197947799No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT7260.101630141876238No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1750.066.285499
GCGAAAG1750.049.714122
GTCTATA1151.4095349E-744.1395531
AGCGAAA2300.037.8339041
AGACGCG1752.4265319E-937.2882145
CGAAAGC2800.033.66063
GAGTGAT3950.033.0378657
TAGAGTG4750.032.052525
AGAACCG1255.1524874E-428.99995
GTCCTAT6800.027.7263161
TTAGAGT5000.026.099914
CTATAGT1701.0309127E-425.5881484
TCTATAG2001.06494135E-525.3749143
TCCTATG7500.025.1332472
GTATATA3601.8371793E-1024.1716611
GTGCTTA2701.6475497E-724.1665869
CAACTAA3102.415436E-823.3870189
TATGATG8150.023.1287575
CCCTGTA3803.619789E-1022.896263145
GTACTGT2852.7765782E-722.894666
Produced by FastQC (version 0.11.5)