FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1975-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1975-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073638
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC13060.1216424902993374No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA11550.10757815949137418No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA11490.10701931190960082No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG11370.10590161674605407No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11100.1033868026280739No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG400.006051699654.3734251
TAGTCGG1604.591311E-836.248958
TATACTG5300.035.565015
GTTACGT1551.4654706E-632.740991
TTACGTG1703.0126648E-629.852082
GTATGAC3201.8189894E-1229.4522723
AAGGTCG1000.006075511728.999169
GCGAAAG3950.027.5308512
AGCGAAA3950.027.5308511
GTTGACA10500.025.546881
CAGGACG2001.065803E-525.3742664
AGTCCGC2301.1026241E-625.21901145
GTTATGT7800.025.0954281
ACCGTGC1450.00122670924.9992778
CTACATC4101.8189894E-1224.7553833
CCGCTCT4600.023.6406214
TACGTGT2151.8663202E-523.603973
GTAATAT4505.456968E-1222.5549031
ACATGGT11350.022.356185
ACACCTA13100.022.1367651
Produced by FastQC (version 0.11.5)