FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1975-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1975-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606472
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC300.001935430272.4994668
AACCGTA400.006049918554.37467
ACGCCGC604.941701E-448.336964145
GTGATCG1354.967369E-737.5923169
GTTACGT800.002033586836.25871
GCGAAAG3200.036.255712
TATACTG3950.033.0404555
CGAAAGC3650.031.7832093
AGCGAAA3700.031.3588751
GTATAAG2151.8037099E-830.3561211
CGACTAG1606.8202906E-527.189546
GTATTAT6400.026.0609421
CTAGGAC3001.7029379E-824.1684823
TATAAGT3052.0296284E-823.7722764
GAGTGAT3102.4141627E-823.3869237
TAGTACC2903.2818753E-722.4998329
GTAATAT2653.7680038E-621.8920461
TATATAC3055.329166E-721.3950483
GTATCTA1750.003652011320.7192571
GGACCAT4601.7644197E-1020.490676
Produced by FastQC (version 0.11.5)