FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1975-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1975-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856806
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG12330.14390655527622356No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11660.13608681545180587No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA10220.11928021045604256No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA9420.1099432076806185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATGT6500.042.384261
TATACTG4350.038.3352475
TGCACCG951.0284652E-438.15985
AGCGAAA3200.036.2496951
GCGAAAG3450.033.6248662
GTTACGT1751.0089752E-733.142581
TTACGTG1801.2914643E-732.223832
TATGTGT8900.031.771253
TTATGTG9550.028.0904082
ATACGGC1306.480294E-427.8860077
AAGACGA1050.007705967427.6204262
TACTGAA11700.027.2663177
TGTTGGC14500.027.0013477
TTAGGTA1907.151095E-626.711864
CTACTGA11700.026.646636
GTTGACA7950.026.4463181
GTAATAT3306.184564E-1126.3634131
ACGTAAC1409.990509E-425.894153
TGTGTTG10650.025.8698355
TAGCATG10100.025.8428752
Produced by FastQC (version 0.11.5)