FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1975-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1975-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800678
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1152.4829205E-950.4345059
AGCGAAA3300.035.153511
GAGCGCG850.002741735734.1174559
CGAAAGC3450.033.62513
TATACTG3450.031.5235315
GCGAAAG3800.030.5280532
AGTCCGC1453.4896675E-529.999832145
CGACACA1000.006072055529.0016526
ACCGTGC1100.00967071926.3634918
GAGCGAA2259.0992035E-725.7792421
ATTGGCG1851.8295168E-423.5148533
CATTACC5450.022.6159653
GTTATGT5300.021.8880371
CTGTGCG2404.3616754E-521.1457169
GTATTAT8850.020.481391
GTCTAGA2908.242063E-620.0011391
ATTAGGT3301.1367792E-619.7738513
GTATTAG1850.00504435419.595711
ACCTCTA6300.019.5633837
TACAGCC7300.018.8697577
Produced by FastQC (version 0.11.5)